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Filament Identification...
Dichotomous Key for Filamentous Microorganism "Identification" in Activated Sludge...
Observed and recorded filament characteristics are used to
characterize the filamentous microorganisms by genus or by a
numbered type using the dichotomous key presented here. This key
lists the 22 filamentous bacteria most commonly observed in
activated sludge. To simplify this key, several filamentous
microorganisms having readily identifiable specific characteristics
are not listed. These include; fungi, Cyanophyceae,
Flexibacter spp. and Bacillus spp. In addition, some
filamentous microorganisms observed only occasionally in activated
sludge are not included in the key. These include Type 1702, 1852,
and 0211.
The use of this key is not without risk because some filamentous
microorganism characteristics vary, and the key cannot always address
all of these variables. The filament type arrived at using the key
should be carefully checked against the typical microorganisms
characteristics listed in table given below.
| Filament type |
1 |
2 |
3 |
4 |
5 |
6 |
7 |
8 |
9 |
10 |
11 |
12 |
13 |
14 |
| S.natans |
- |
- |
- |
- |
- |
PHB |
1.0-1.4 |
500 |
St |
E |
+ |
+ |
+ |
- |
| Type 1701 |
- |
- |
- |
- |
- |
PHB |
0.6-0.8 |
20-80 |
St-B |
I-E |
+ |
+ |
+ |
++ |
| Type 0041 |
+,V |
- |
-,+ |
- |
- |
- |
1.4-1.6 |
100-500 |
St |
I-E |
+ |
- |
+ |
++,- |
| Type 0675 |
+,V |
- |
-,+ |
- |
- |
- |
0.8-1.0 |
50-150 |
St |
I |
+ |
- |
+ |
++,- |
| Type 021N |
- |
- |
-,+ |
-,+ |
+ |
PHB |
1.0-2.0 |
50-500 |
St-SC |
E |
+ |
+ |
- |
- |
| Thiothrix I |
-,+ |
- |
-,+ |
+,- |
+ |
PHB |
1.4-2.5 |
100-500 |
St-SC |
E |
+ |
- |
+ |
- |
| Thiothrix II |
- |
- |
-,+ |
+,- |
+ |
PHB |
0.8-1.4 |
50-200 |
St-SC |
E |
+ |
- |
+ |
- |
| Type 0914 |
-,+ |
- |
-,+ |
-,+ |
- |
PHB |
1.0 |
50-200 |
St |
E-F |
+ |
- |
- |
- |
| Beggiatoa |
-,+ |
- |
-,+ |
+,- |
+ |
PHB |
1.2-3.0 |
100-500 |
St |
F |
-+ |
- |
- |
- |
| Type 1851 |
+ |
? |
- |
- |
- |
- |
0.8 |
100-300 |
St-B |
E |
+- |
- |
+ |
-,+ |
| Type 0803 |
- |
- |
- |
- |
- |
- |
0.8 |
50-150 |
St |
E-F |
+ |
- |
- |
- |
| Type 0092 |
- |
+ |
- |
- |
- |
+ |
0.8-1.0 |
20-60 |
St-B |
I |
+- |
- |
- |
- |
| Type 0961 |
- |
- |
- |
- |
- |
- |
0.8-1.2 |
40-80 |
St |
E |
+ |
- |
- |
- |
| M.parvicella |
+ |
- |
+ |
- |
- |
PHB |
0.8 |
100-400 |
C |
I |
- |
- |
- |
- |
| Nocardia |
+ |
- |
+ |
- |
- |
PHB |
1.0 |
10-20 |
I |
I |
+- |
- |
- |
- |
| N.limicola I |
+ |
+ |
- |
- |
- |
- |
0.8 |
100 |
C |
I-E |
- |
- |
- |
- |
| N.limicola II |
-,+ |
+,- |
- |
- |
- |
PHB |
1.2-1.4 |
100-200 |
C |
I-E |
+ |
+ |
- |
- |
| N.limicola III |
+ |
+ |
- |
- |
- |
PHB |
2.0 |
200-300 |
C |
I-E |
+ |
+ |
- |
- |
| H.hydrossis |
- |
- |
- |
- |
- |
- |
0.5 |
20-100 |
St-B |
E-F |
- |
- |
+ |
-,+ |
| Type 0581 |
- |
- |
- |
- |
- |
- |
0.5-0.8 |
100-200 |
C |
I |
- |
- |
- |
- |
| Type 1863 |
- |
- |
-,+ |
- |
- |
- |
0.8 |
20-50 |
B-I |
E-F |
+ |
+ |
- |
- |
| Type 0411 |
- |
- |
- |
- |
- |
- |
0.8 |
50-150 |
B-I |
E |
+ |
+ |
- |
- |
|
1 : Gram stain, 2 : Neisser stain (trichome), 3 : Neisser stain
(granules), 4 : S granules (in - situ), 5 : S granules (S test), 6 :
Other cell inclusions, 7 : Trichome diameter, 8 : Trichome length,
9 : Trichome shape, 10 : Trichome location, 11 : Cell septa clearly
observed, 12 : Indentations at cell septa, 13 : Sheath, 14 : Attached
growth
|
|
+ : Positive, - : Negative, V : Variable, +,- or -,+ : Variable, the
first being most observed, St : Straight, B : Bent, SC : Smoothly curved,
C : Coiled, I : Irregularly shaped, E : Extends from floc surface, F :
Free in liquid between the flocs
|
"Correlation Between Filament Type Occurence and AS Plant Conditions or Wastewater Types"...
"Effect of Wastewater Origin on SVI & on Dominating Filamentous Microorganisms"...
"Specific Nutrient Requirements for BOD Removal"...
If characteristics given in the table shown above do not correspond
to the filament type arrived at using the key, careful re - examination
of characteristics used in the key is in order. For example, Type 0041
generally gives a weak Gram positive or a Gram variable reaction, and
as such, is keyed correctly from the identification procedure given
here. However, if strongly Gram positive, it would be keyed as
N. limicola - II. Reference to table given above shows
N. limicola - II to be coiled and to possess no attached growth
- Type 0041 is straight or smoothly - curved and most often has substantial
attached growth.
Occasionally, a filamentous microorganism is observed that is not
represented by a type or genus designation. Such a filamentous
microorganism should be reported as "not identified"; do not
try to "force - fit" the microorganism into existing filament
types.
"Dichotomous Key for Filamentous Microorganism Identification in Activated Sludge"...
( PS : Please use the button of "Expand to regular size"... )
Do You Want to Identify Your Lovely ( ! ) Filamentous Bacteria...?
Here is the Key...
Do You Want to Get More Info About the Filamentous Bacteria...?
Here is the Info...
Do You Want to Get a Good Lecture About the Filamentous Bacteria...?
Here is the Info...
Do You Want to Get More Info About the Other Bacteria...?
Here is the Info...
Do You Want to Get a Huge Archives of the Bacteria...?
Here is the Archives...
