You Have Found The Satan...!


Hello...! My Name is Type 0914...!

Characteristics : Straight or smoothly - curved filaments, 0.7 - 1.0 micron in width and 50 - 200 micron in length, found extending from the floc surface or more commonly free in the bulk solution. May have incidental attached growth. Cells are square - shaped (1.0 x 1.0 micron) without constrictions at septa. No sheath is present. Gram negative and Neisser negative, but may stain Gram positive when substantial sulfur granules are present. Neisser positive granules may occur. May contain intracellular sulfur granules, which appear square rather than spherical as observed for other filamentous microorganisms.

Relatively small, non-motile filaments (50-200 µm). Straight or smoothly curved filaments with no branching. Cells are square (0.7 x 1.0 µm). No sheath, no constrictions at the septa. Filaments are found extending from the floc structure and free in the bulk solution. May contain intracellular sulfur granules. Usually easy to identify due square sulfur granules as opposed to spherical on other types of filaments under phase contrast. Does not respond to the S test. Poly-ß-hydroxybutric acid (PHB) is frequently observed as dark intracellular granules. May have incidental attached growth. The filament staining is Gram negative or Gram positive when sulfur granules present and Neisser negative with Neisser positive granules observed frequently.

Similar Organisms : Types 1851 and Type 0803 are similar although Type 0914 occurs in bundles, and neither Types 1851 or Type 0803 have sulfur granules. Thiothrix is similar also.

Environment : This sulfur oxidizing filament is usually found in environments where there are septic wastes and sulfides. It is commonly observed in activated sludge but rarely causes bulking. This filament is usually found in environments where there low F/M (0.02-0.2) with simple sugars and organic acids. N deficiency may also be a cause.

Control : F/M can be changed by increased sludge wasting, changing from complete mix to plug flow or use of a selector. Increasing N levels has been shown to reduce bulking. Septic wastes can be altered by pre-aeration or pre-chlorination.

Rank : Type 0914 ranks 18th in number of predominance.