You Have Found The Satan...!
Characteristics : Straight or smoothly - curved filaments, 0.7 - 1.0 micron in width and 50 - 200 micron in
length, found extending from the floc surface or more commonly free in the bulk solution. May have incidental attached
growth. Cells are square - shaped (1.0 x 1.0 micron) without constrictions at septa. No sheath is present. Gram
negative and Neisser negative, but may stain Gram positive when substantial sulfur granules are present. Neisser
positive granules may occur. May contain intracellular sulfur granules, which appear square rather than spherical
as observed for other filamentous microorganisms.
Relatively small, non-motile filaments (50-200 µm). Straight or smoothly curved filaments with no branching. Cells
are square (0.7 x 1.0 µm). No sheath, no constrictions at the septa. Filaments are found extending from the floc
structure and free in the bulk solution. May contain intracellular sulfur granules. Usually easy to identify due
square sulfur granules as opposed to spherical on other types of filaments under phase contrast. Does not respond
to the S test. Poly-ß-hydroxybutric acid (PHB) is frequently observed as dark intracellular granules. May have
incidental attached growth. The filament staining is Gram negative or Gram positive when sulfur granules present
and Neisser negative with Neisser positive granules observed frequently.
Similar Organisms : Types 1851 and Type 0803 are similar although Type 0914 occurs in bundles, and neither
Types 1851 or Type 0803 have sulfur granules. Thiothrix is similar also.
Environment : This sulfur oxidizing filament is usually found in environments where there are septic wastes
and sulfides. It is commonly observed in activated sludge but rarely causes bulking. This filament is usually found
in environments where there low F/M (0.02-0.2) with simple sugars and organic acids. N deficiency may also be a cause.
Control : F/M can be changed by increased sludge wasting, changing from complete mix to plug flow or use of
a selector. Increasing N levels has been shown to reduce bulking. Septic wastes can be altered by pre-aeration or
pre-chlorination.
Rank : Type 0914 ranks 18th in number of predominance.