You Have Found The Satan...!
Characteristics : Very similar to Type 0041, only smaller in trichome length (50 - 150 micron) and cell dimension
(0.7 - 1.0 micron). Covered with heavy, attached growth in domestic waste activated sludge; may lack attached growth in
some industrial waste activated sludge. A sheath is present. Gram positive to Gram variable, Neisser negative. Neisser -
positive granules occur; no sulfur granules.
Identification : Type 0675 is a relatively small, non-motile filaments (50-150 µm). Straight, smoothly curved or
bent filaments with no branching can be found on this filament.. Cells are square (0.7-1.0 x 1.0 µm) and contained in a
tight fitting sheath. No indentations at septa. Filaments are found within the floc structure causing open, lacy floc or
radiating outwards causing inter-floc bridging. The filament staining is Gram positive or Gram variable when in the floc
structure and Neisser negative with Neisser positive granules observed infrequently. Neisser positive slime coat frequently
observed in industrial wastes. Intracellular granules rarely observed. No sulfur granules. Usually heavy attached growth
is present when observed inside the floc structure and sometimes no attached growth when extending into the bulk solution.
Sheath is difficult to detect except when missing cells or with high powered microscopes or digital cameras.
Similar Organisms : Type 0041 is nearly identical although larger in diameter. Type 1701 can be similar although it
is gram negative and the cells are round ended. Type 1851 is similar although it occurs in bundles and doesn’t have as much
attached growth. Type 0914 and Thiothrix are similar although these two have intracellular sulfur granules and don’t have
attached growth. Type 0675 is smaller than Type 0041 and both can have heavy attached growth in an older sludge. But you
can also see Type 1851 in the gram stain photo, even smaller than 0675.
Environment : This filament is usually found in environments where there low F/M (0.02-0.2) and with a long MCRT
(10-40 days). Nitrogen or Phosphorous deficiency may also be a cause.
Control : F/M can be changed by increased sludge wasting, changing from complete mix to plug flow or use of a
selector. RAS Chlorination should be definitely used if in the rapid growth phase. Increasing N and P levels has been
shown to reduce bulking. Nutrient deficient wastes can be checked by effluent values of residual NH3 and
o-PO4 and should be supplemented if necessary.
Rank : Type 0675 ranks 10th in number of predominance. Common in sludge in low abundance. Can be
beneficial at low abundance as a backbone structure for the floc.